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Small 2012, 8, 3000-3008, We described a method for the real-time and high-throughput monitoring of the self-assembly and disassembly of complex DNA superstructures, using temperature-dependent Förster resonance energy transfer (FRET) spectroscopy. 1, pp. Niemeyer*. Hierarchical assembly of DNA filaments with designer elastic ptoperties. 10 (2019) Nr. Search for more papers by this author. Network. Niemeyer. 8, pp. Notes. 192 - 204, Sacca, Barbara; Renner, Christian; Moroder, Luis, The chain register in heterotrimeric collagen peptides affects triple helix stability and folding kinetics, In: Journal of Molecular Biology Vol. 49 (2010) Nr. 3 (2020) Nr. 12, pp. 2 1. barbara.sacca@uni-due.de; Centre for Medical Biotechnology (ZMB), University of Duisburg–Essen, Universitätstrasse 2, 45117 Essen, Germany . Their combined citations are counted only for the first article. Author information: (1)Centre for Medical Biotechnology (ZMB) and Centre for Nano Integration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitätstr. Wie das funktioniert, erforscht Dr. Barbara Saccà. Nom à l'état civil. Saccà lab for Bionanotechnology Prof. Dr. Barbara Saccà. Contact. 42 (2003) Nr. 0201/18 3-3395, barbara.sacca@uni-due.de Redaktion: Cathrin Becker, Tel. From atoms to molecules and bio‐macromolecules, from organelles to cells, tissues, to the whole living system, nature shows us that the formation of complex systems with emergent properties originates from the hierarchical self‐assembly of single components in guided bottom‐up processes. We used a DNA-based self-assembly method for the construction of switchable DNA devices based on G-quadruplex moieties, which are patterned on quasi-planar DNA arrays with nanoscale precision. Information (par souci de protection des données à caractère personnel, le jour et le mois de naissance ne sont pas affichés) Langue d'expression : Anglais. High-throughput, realtime monitoring of the self-assembly of DNA nanostructures by FRET spectroscopy. 2, 45117, Essen, Germany. 12, pp. Mikayel Aznauryan. Bionanotechnology 7 (2011) Nr. 1, pp. 2009, 4, 271-285. 5, pp. In this work, we show that the assembly pathway leading to oligomeric chains can be finely tuned and fully controlled, enabling to emulate the formation of protein-like filaments using a single construction principle. To this end, two oligonucleotides out of the entire set building up the superstructure were labeled with a fluorescein and a tetramethylrhodamine, as FRET donor and acceptor, respectively. Reads . 5 Jobs sind im Profil von Dr. Barbara Sacca aufgelistet. 713 - 724, De Cian, Anne; Guittat, Lionel; Kaiser, Markus; Sacca, Barbara; Amrane, Samir; Bourdoncle, Anne; Alberti, Patrizia; Teulade-Fichou, Marie-Paule; Lacroix, Laurent; Mergny, Jean-Louis, Fluorescence-based melting assays for studying quadruplex ligands, In: Methods: A Companion to Methods in Enzymology Vol. About. Enzyme-functionalized DNA nanostructures as tools for organizing and controlling enzymatic reactions - Volume 42 Issue 12 - Guido Grossi, Andreas Jaekel, Ebbe Sloth Andersen, Barbara Saccà 2009, pp. 2009-2012 Principal Investigator at the Technische Universität Dortmund, Germany (funded by the Deutsche Forschungsgemeinschaft, grant SA 1952/1-1) 2006 – 2009 Postdoc with C.M. 49, pp. Altogether, this study confirmed that DNA superstructures are well-suited scaffolds for accommodation of mechanically switchable units and thus opens the door to the development of more sophisticated nanomechanical devices. About. 8 (2002) Nr. 2008, 47, 2135-2137. Sacca, Barbara; Meyer, Rebecca; Niemeyer, Christof M. Analysis of the Self-Assembly of 4×4 DNA Tiles by Temperature-Dependent FRET Spectroscopy, In: ChemPhysChem Vol. 183 - 195, Conti, Chiara; Sacca, Barbara; Herrick, John; Lalou, Claude; Pommier, Yves; Bensimon, Aaron, Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells, In: Molecular Biology of the Cell Vol. 8 (2002) Nr. 54 (2015) Nr. 33, pp. Sites of high local frustration in DNA origami. 12, pp. B. Saccà, B. Siebers, R. Meyer, M. Bayer and C.M. In: Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry Vol. Chem. 7. 23539 - 23547, Grossi, Guido; Jaekel, Andreas; Andersen, Ebbe Sloth; Sacca, Barbara, Enzyme-functionalized DNA nanostructures as tools for organizing and controlling enzymatic reactions, In: MRS Bulletin Vol. 11, pp. Barbara Saccà ZMB, University Duisburg-Essen Verified email at uni-due.de. *Post-doctoral fellowship in DNA nanotechnology* A post-doctoral fellowship is available in the Saccà laboratory for Bionanotechnology at the Center for Medical Biotechnology of the University of Duisburg-Essen, Germany. 13 (2017) Nr. 324 (2002) Nr. 2135 - 2137, Rao, V. Ashutosh; Conti, Chiara; Guirouilh-Barbat, Josee; Nakamura, Asako; Miao, Ze-Hong; Davies, Sally L.; Sacca, Barbara; Hickson, Ian D.; Bensimon, Aaron; Pommier, Yves, Endogenous γ-H2AX-ATM-Chk2 checkpoint activation in Bloom's syndrome helicase-deficient cells is related to DNA replication arrested forks, In: Molecular Cancer Research Vol. Saccà B(1). B. Saccà*, R. Meyer and C.M. Universitätsstraße 2 319 (2002) Nr. W2-professorship at the University of Duisburg-Essen: Since 04.2012: Group leader at the University of Duisburg-Essen: 2009 - 2012: Principal Investigator at the Technische Universität Dortmund, Germany: … 9378 - 9383. 7582 - 7597, From Nano to Macro through Hierarchical Self-Assembly : The DNA Paradigm, In: ChemBioChem Vol. E.-C. Schöneweiß and B. Saccà. 0203/37 9-1488, cathrin.becker@uni-due.de Alle Storys 1063 - 1080, Poepsel, Simon; Sprengel, Andreas; Sacca, Barbara; Kaschani, Farnusch; Kaiser, Markus; Gatsogiannis, Christos; Raunser, Stefan; Clausen, Tim; Ehrmann, Michael, Determinants of amyloid fibril degradation by the PDZ protease HTRA1, In: Nature Chemical Biology Vol. barbara.sacca@uni … 3000 - 3008, Covalent Tethering of Protruding Arms for Addressable DNA Nanostructures, In: Small Vol. *Post-doctoral fellowship in DNA nanotechnology* A post-doctoral fellowship is available in the Saccà laboratory for Bionanotechnology at the Center for Medical Biotechnology of the University of Duisburg-Essen, Germany. E-mail address: barbara.sacca@uni‐due.de. Prof. Dr. Barbara Saccà ist neu an der Uni Duisburg-Essen. 862 - 869. barbara.sacca@uni-due.de Centre for Medical Biotechnology (ZMB), University of Duisburg–Essen, Universitätstrasse 2, 45117 Essen, Germany Search for more papers by this author PMID: 31615123 PMCID: PMC6832416 DOI: 10.3390/molecules24203694 Abstract Nucleic … *Please note: The data privacy settings related to this external link are subject to the respective third-party's privacy policies, terms and conditions: Verified email at iecb.u-bordeaux.fr - Homepage. 18 (2007) Nr. 2009-2012 Principal Investigator at the Technische Universität Dortmund, Germany (funded by the Deutsche Forschungsgemeinschaft, … Arbeitsgruppen der Fakultät für Biologie der Universität Duisburg-Essen 28, pp. 45141 Essen, Campus Essen Sehen Sie sich auf LinkedIn das vollständige Profil an. 1,249. About. 2014, pp. 3 (2002) Nr. Covalent tethering of protruding arms for addressable DNA nanostructures. Entropy-driven folding and nucleation seeds formation may provide possible solutions; however, until now, a unified view of the energetic factors in play is missing. ); pierre.stegemann@uni-due.de (P.S.) Niemeyer, TU Dortmund. 4486 - 4496, Streich, Carmen; Akkari, Laura; Decker, Christina; Bormann, Jenny; Rehbock, Christoph; Müller-Schiffmann, Andreas; Niemeyer, Felix; Nagel-Steger, Luitgard; Willbold, Dieter; Sacca, Barbara; Kor, Carsten, Characterizing the Effect of Multivalent Conjugates Composed of Aβ-Specific Ligands and Metal Nanoparticles on Neurotoxic Fibrillar Aggregation, In: ACS Nano (2016) pp. Nucleic acids nanotechnology. This new and rapidly emerging field of science makes it possible to construct three-dimensional DNA objects of almost any desired size and shape and, most importantly, with programmable nanoscaled features. By continuing to browse this site you agree to us using cookies as described in About Cookies.. Abstract Today, DNA nanotechnology is one of the methods of choice to achieve spatiotemporal control of matter at the nanoscale. Remove maintenance message Whereas the flexibility of the intra-domains region can be regulated by switchable DNA motifs, the inter-domain interfaces feature mutually and self-complementary shapes, whose pairwise association leads to filaments of programmable periodicity and variable persistence length. The complexity of natural macromolecular assemblies is normally achieved through a hierarchy paradigm, which mostly relies on step-wise interactions between shape-complementary subunits. Nanolattices of switchable DNA-based motors. 47, pp. 333 (2004) Nr. Saccà lab for Bionanotechnology ... barbara.sacca@uni-due.de. 4 (2006) Nr. 7, pp. Nucleic acids and proteins are two major classes of biopolymers in living systems. 16 - 19, Sacca, Barbara; Sprengel, Andreas; Feldkamp, Udo, De novo design of nucleic acid structures, In: De novo Molecular Design Schneider, Gisbert; (Eds.) 12 (2018) Nr. 5, pp. 25 (2020) Nr. barbara.sacca@uni-due.de. Angew Chem Int Ed Engl. Whereas nucleic acids are characterized by robust molecular recognition properties, essential for the reliable storage and transmission of the genetic information, the variability of structures displayed by proteins and their adaptability to the environment make them ideal functional materials. 11, pp. Die neue Professorin für DNA-Technologie untersucht derzeit vor allem seinen molekularen Aufbau am Zentrum für Medizinische Biotechnologie (ZMB) der Uni … 4 (2009) Nr. 42 (2017) Nr. 45141 Essen, Jaekel, Andreas; Lill, Pascal; Whitelam, Stephen; Sacca, Barbara, Insights into the Structure and Energy of DNA Nanoassemblies, In: Molecules Vol. 734. 81 - 94, Amrane, Samir; Sacca, Barbara; Mills, Martin; Chauhan, Madhu; Klump, Horst H.; Mergny, Jean-Louis, Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats, In: Nucleic Acids Research Vol. 14472, Schöneweiß, Elisa-Charlott; Sacca, Barbara, The collective behavior of spring-like motifs tethered to a DNA origami nanostructure, In: Nanoscale Vol. Structural reconfiguration allows complex systems to perform different tasks depending on environmental conditions and therefore to switch on/off critical functions. Publications. 48 (2009) Nr. Barbara Saccà, Center for Nanointegration Duisburg‐Essen (CENIDE) and Center for Medical Biotechnology (ZMB), University of Duisburg‐Essen, Universitätsstrasse 2, 45117 Essen … 4065 - 4077, Sacca, Barbara; Lacroix, Laurent; Mergny, Jean-Louis, The effect of chemical modifications on the thermal stability of different G-quadruplex-forming oligonucleotides, In: Nucleic Acids Research Vol. *Project* The project belongs to a large initiative including different research groups of the University of Duisburg-Essen, whose final goal is to establish 4, pp. andreas.jaekel@uni-due.de. 58 - 66, Tennstaedt, Annette; Pöpsel, Simon; Trübestein, Linda; Hauske, Patrick; Brockmann, Anke; Schmidt, Nina; Irle, Inga; Sacca, Barbara; Niemeyer, Christof M.; Brandt, Roland; Ksiezak-Reding, Hanna; Tirniceriu, Anca Laura; Egensperger, Rupert; Baldi, Alfonso; Dehmelt, Leif; Kaiser, Markus; Huber, Robert; Clausen, Tim; Ehrmann, Michael, Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates, In: Journal of Biological Chemistry Vol. Prof. Dr. Barbara Saccà ist neu an der Uni Duisburg-Essen. Parerga und Paralipomena; Arthur Schopenhauer Dedicated to my family . Schöneweiß EC(1), Saccà B(1). Manipulating Enzymes Properties with DNA Nanostructures. 33 (2005) Nr. 40 (2011) Nr. 9 (2017) Nr. Reads . B. Saccà,* Y. Ishitsuka, R. Meyer, A. Sprengel, E. C. Shöneweiß, U. Nienhaus,* C. M. We show that the organized scaffolding of multiple hairpins enhances the thermal stability of the device and that the coordinated action of the tethered motors can be used to mechanically unfold a G-quadruplex motif bound into the inner cavity of the origami structure, thus surpassing the operational capabilities of freely diffusing motors. We are currently employing DNA origami technology for the construction of artificial DNA-based nanocontainers, which mimic the shape and function of natural compartmentalization systems. Post-Doc +49 201 183 6911 T03 R01 D27. 11 (2015) Nr. 10 (2018) Nr. Arbeitsgruppe Mikrobiologie II. Nat. barbara.sacca@uni-due.de. The self-assembly of a DNA origami structure, although mostly feasible, represents indeed a rather complex folding problem. 3, pp. Universitätsstr. 920 - 924, Preisler, Zdeněk; Sacca, Barbara; Whitelam, Stephen, Irregular model DNA particles self-assemble into a regular structure, In: Soft Matter Vol. 2, pp. Pierre STEGEMANN of University of Duisburg-Essen, Essen (uni-due) | Read 4 publications | Contact Pierre STEGEMANN 45117 Essen, Germany *barbara.sacca@uni-due.de This chapter is a clear introduction to the role of DNA molecule in Nanotechnology. 1235 - 1242, Synthesis of heterotrimeric collagen peptides containing the α 1 β 1 integrin recognition site of collagen type IV, In: Journal of Peptide Science Vol. geboren in Essen Zentrum für Medizinische Biotechnologie und Fakultät der Biologie der Universität Duisburg-Essen 2017 "In Hinsicht auf die Schätzung der Größe eines Menschen gilt für die geistige das umgekehrte Gesetz der physischen: Diese wird durch die Ferne verkleinert, jene vergrößert." Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions. Barbara Saccà's 60 research works with 2,152 citations and 5,113 reads, including: Insights into the Structure and Energy of DNA Nanoassemblies Engl. 399 (2018) Nr. 22. 18, pp. 73 - 77, In: Methods: A Companion to Methods in Enzymology Vol. The reversible switching of the devices was triggered by addition of DNA sequences ('fuels') and translated into linear extension/contractile movements. 20.02.2017: Winzige Nano-Container aus DNA-Material zu konstruieren, die passgenau jeweils ein ganz bestimmtes Protein binden können - dies gelang jetzt der Nachwuchswissenschaftlerin Dr. Barbara Sacca von der Universität Duisburg-Essen … From atoms to molecules and biomacromolecules, from organelles to cells, tissues, till the whole living system: so nature shows us that the realization of complex systems with emergent properties originates from the hierarchical self-assembly of single components in a guided bottom-up process. Niemeyer*. 3239 - 3248, Feldkamp, Udo; Niemeyer, Christof M.; Sacca, Barbara, Dendritic DNA Building Blocks for Amplified Detection Assays and Biomaterials, In: Angewandte Chemie International Edition Vol. Nat. 3694, Kosinski, Richard; Mukhortava, Ann; Pfeifer, Wolfgang; Candelli, Andrea; Rauch, Philipp; Sacca, Barbara, Sites of high local frustration in DNA origami, In: Nature Communications Vol. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Pays : pays inconnu. 1, pp. The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. 54 (2015) Nr. We thus propose a dynamic model of DNA origami assembly that relies on the capability of the system to escape high structural frustration at nucleation sites, eventually resulting in the emergence of a more favorable but previously hidden state. Another important aspect of biological self-assembling systems, to which we are inspired, is the capability to change their shape in response to an external event. We aim at using such artificial DNA devices for selective loading/release of proteins and envisage potential applications in biosensing as well as cargo transport and delivery. Kosinski R(1), Mukhortava A(2), Pfeifer W(1), Candelli A(2), Rauch P(2), Saccà B(3). Meyer, Rebecca; Sacca, Barbara; Niemeyer, Christof M. Site-Directed, on-surface assembly of DNA nanostructures, In: Angewandte Chemie International Edition Vol. 45141 Essen, Travelling the energy landscape of DNA origami folding, DNA filaments with different ultrastructures and persistence lengths, Rational design of DNA hosts for protein guests, Hierarchical self-assembly of DNA nanostructures, Reversible reconfiguration of a planar DNA cage followed by single-molecule FRET, Monitoring the self-assembly of DNA nanostructures in real time by FRET spectroscopy. 865 - 866, Jaekel, Andreas; Stegemann, Pierre; Saccà, Barbara. Chembiochem. University Duisburg-Essen. Die neue Professorin für DNA-Technologie untersucht derzeit vor allem seinen molekularen Aufbau am Zentrum für Medizinische Biotechnologie (ZMB) der Uni Duisburg-Essen … Wie das funktioniert, erforscht Dr. Barbara Saccà. Methods. Commun. Correspondence and requests for materials should be addressed to B.S. 33 (2005) Nr. 8 (2012) Nr. ‪ZMB, University Duisburg-Essen‬ - ‪Cited by 3,105‬ - ‪Bionanotechnology‬ The following articles are merged in Scholar. 773 - 785, Daems, Devin; Pfeifer, Wolfgang; Rutten, Iene; Sacca, Barbara; Spasic, Dragana; Lammertyn, Jeroen, Three-Dimensional DNA Origami as Programmable Anchoring Points for Bioreceptors in Fiber Optic Surface Plasmon Resonance Biosensing, In: ACS Applied Materials & Interfaces Vol. Contact +49 201 183 -4640 +49 201 183 -3672 ... IMCES Imaging Center University Hospital Essen … 216 - 224, Renner, Christian; Sacca, Barbara; Moroder, Luis, Synthetic Heterotrimeric Collagen Peptides as Mimics of Cell Adhesion Sites of the Basement Membrane, In: Biopolymers - Peptide Science Section Vol. 2, pp. Dr. Barbara Saccà, Zentrum für Medizinische Biotechnologie, Tel. We use switchable DNA motifs and integrate them within static DNA nanostructures to achieve control of matter distribution both in space and time. 2, 45117, Essen, Germany. Independent research position at the University of Duisburg-Essen. Google. Universitätsstraße 2 ACS Nano. 45141 Essen, Campus Essen 7, pp. 51 (2012) Nr. Correct assembly of the superstructure was monitored by temperature-dependent FRET efficiency. Publications. 5996 - 6000, Sacca, Barbara; Niemeyer, Christof M.; Meyer, Rebecca, Temperature-dependent FRET spectroscopy for the high-throughput analysis of self-assembled DNA nanostructures in real time, In: Nature protocols Vol. 3429 - 3436, Sacca, Barbara; Sinner, Eva-Kathrin; Kaiser, Jens; Lübken, Christoph; Eble, Johannes A.; Moroder, Luis, Binding and docking of synthetic heterotrimeric collagen type IV peptides with α1β1 integrin, In: ChemBioChem Vol. Protoc. ‪ZMB, University Duisburg-Essen‬ - ‪Cited by 3,161‬ - ‪Bionanotechnology‬ The following articles are merged in Scholar. 44 - 55, Synthetic DNA filaments : from design to applications, In: Biological Chemistry Vol. We use switchable DNA motifs and integrate them within the central seam of a DNA origami structure to reversibly enlarge and contract its inner cavity for modulation of fluorescence energy transfer between two internalized fluorophores and selective trapping of proteins. Entropy-driven folding and nucleation seeds … Monitoring the self-assembly of DNA nanostructures in real time by FRET spectroscopy, B. Saccà*, R. Meyer, U. Feldkamp, H. Schroeder and C.M. 13, pp. 309 - 318, Daems, Devin; Rutten, Iene; Pfeifer, Wolfgang; Decrop, Deborah; Spasic, Dragana; Bath, Jonathan; Sacca, Barbara; Turberfield, Andrew; Lammertyn, Jeroen, DNA origami nanostructured surfaces for enhanced detection of molecular interactions, In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018): Kaohsiung, Taiwan, 11-15 November 2018 ; Vol. Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, Aarhus 8000, Denmark. Barbara Saccà's 59 research works with 2,106 citations and 4,838 reads, including: Sites of high local frustration in DNA origami 67 (2014) Nr. 42 (2007) Nr. 1, pp. Follow. Andreas Jaekel, Pierre Stegemann and Barbara Saccà * ZMB, University Duisburg-Essen, Universitätstraße 2, 45117 Essen, Germany; andreas.jaekel@uni-due.de (A.J. Group Leader at European Institute of Chemistry and Biology (IECB), University of Bordeaux. 47 (2008) Nr. * Reversible reconfiguration of DNA origami nanochambers monitored by single-molecule FRET. Center for Nanointegration Duisburg‐Essen (CENIDE) and Center for Medical Biotechnology (ZMB), University of Duisburg‐Essen, Universitätsstrasse 2, 45117 Essen (Germany) These authors contributed equally to this work. Publications 22. Small 2011, 7, 2887-2898. E-mail address: barbara.sacca@uni-due.de Centre for Medical Biotechnology (ZMB), University of Duisburg–Essen, Universitätstrasse 2, 45117 Essen, Germany Search for more papers by … Commun. About Cookies, including instructions on how to turn off cookies if you wish to do so. (3)ZMB, University of Duisburg-Essen, Universitätstr. Compared with other spectroscopic approaches, such as UV-visible and circular dichroism, the method has advantages in terms of sensitivity, feasibility for high-throughput analysis and applicability to virtually any kind of supramolecular structure. 8894 - 8902, Sprengel, Andreas; Lill, Pascal; Stegemann, Pierre; Bravo-Rodriguez, Kenny; Schöneweiß, Elisa-Charlott; Merdanovic, Melisa; Gudnason, Daniel; Aznauryan, Mikayel; Gamrad; Barcikowski, Stephan; Sanchez-Garcia, Elsa; Birkedal, Victoria; Gatsogiannis, Christos; Ehrmann, Michael; Sacca, Barbara, Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions, In: Nature Communications Vol. Centre for Medical Biotechnology (ZMB), University of Duisburg–Essen, Universitätstrasse 2, 45117 Essen, Germany. Die neue Professorin für DNA-Technologie untersucht derzeit vor allem seinen molekularen Aufbau am Zentrum für Medizinische Biotechnologie (ZMB) der Uni … Sacca, Barbara; Ishitsuka, Yuji; Meyer, Rebecca; Sprengel, Andreas; Schöneweiß, Elisa-Charlott; Nienhaus, G. Ulrich; Niemeyer, Christof M. Reversible reconfiguration of DNA origami nanochambers monitored by single-molecule FRET, In: Angewandte Chemie International Edition Vol.

Männer Outfits Elegant, Drachenhöhle Kommende Veranstaltungen, El Greco Derne Speisekarte, Hazet Assistent Zusammenklappen, Chinesische Suppe Einfach, Business Administration Studium Hamburg, Breidenbacher Hof Teatime, Binomische Formel Rückwärts Rechner,